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Plasmid#83094PurposeGateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tagDepositorInsertSMAD4 (SMAD4 Human)
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5-V5 blast
Plasmid#83100PurposeLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.3
Plasmid#112810PurposePCR template vector for amplifying gRNAcore-pU6.2 promoter fragmentDepositorInsertU6:3-gRNAcore
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mus-Bnip3_deltaExon3
Plasmid#100793PurposeMammalian expression of Bnip3_deltaExon3 splice variantDepositorInsertmouse-Bnip3_deltaExon3 (Bnip3 Mouse)
UseTagsHAExpressionMammalianMutationExon3 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE_AW-pHG165
Plasmid#90062PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW
UseTagsExpressionBacterialMutationI48V/Q55A/Q60RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhS-pHG165a
Plasmid#90040PurposeConstitutive expression of a chimeric LacI:GalS transcription repressorDepositorInsertLLhS
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhF-pHG165a
Plasmid#90041PurposeConstitutive expression of a chimeric LacI:FruR transcription repressorDepositorInsertLLhF
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a
Plasmid#90042PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhR-pHG165a
Plasmid#90043PurposeConstitutive expression of a chimeric LacI:RbsR transcription repressorDepositorInsertLLhR
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a
Plasmid#90044PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE-pHG165
Plasmid#90045PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a
Plasmid#90046PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only