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Showing: 5321 - 5340 of 65821 results

  4. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    170349-plasmid-map-sequence-id-344647

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  5. pCDNA_B2AR-s-pep

    Plasmid
    #47438
    Purpose
    FRET sensor for beta2-adrenergic receptor
    Depositor
    Sivaraj Sivaramakrishnan
    Publication
    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta2-adrenergic receptor (ADRB2 Human)
    Tags
    10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Sept. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  7. pLenti-PGK-EGFP-ALDH1A1-Neo

    Plasmid
    #189742
    Purpose
    The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.
    Depositor
    Robert Sobol
    Publication
    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ALDH1A1 (ALDH1A1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    189742-plasmid-map-sequence-id-374849

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  8. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Publication
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  10. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid
    #169910
    Purpose
    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniCAFE
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    CMV
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169910-plasmid-map-sequence-id-336045

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  12. sfGFP-ER-5

    Plasmid
    #56483
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER (CALR Human)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Publication
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Expression
    Bacterial
    Promoter
    PosmY, PsulA, and grpE
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-520319

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  15. pKMD106h - intein-Cas9(C574)

    Plasmid
    #64191
    Purpose
    Expresses intein-Cas9(C574) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64191-plasmid-map-sequence-id-107550

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  16. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  17. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

    Plasmid
    #193715
    Purpose
    Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-353); mCherry: Met1 Deleted
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193715-plasmid-map-sequence-id-382800

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  18. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  19. pFLAG-CMV2-Hsp27-S78D/S82D

    Plasmid
    #85187
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S78D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    85187-plasmid-map-sequence-id-173286

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Showing: 5321 - 5340 of 65821 results