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pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-SCYL1

Plasmid

#23614

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SCYL1 (SCYL1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-DeltaER

Plasmid

#238416

Purpose

Expresses GRP78 lacking the ER signal peptide in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The first 18 amino acids which is the ER signal p…

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-G227D

Plasmid

#235207

Purpose

Expresses GRP78 with G227D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Glycine 227 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-AID-N51A-CBE N

Plasmid

#227421

Purpose

expresses the N-terminal of AID-N51A-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-AID-N51A-CBE N-terminal (AICDA Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)GCN4-L-GFP

Plasmid

#193714

Purpose

Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-353);

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

Plasmid

#184407

Purpose

yeast surface display of the SARS-CoV-2 Delta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

L452R+T478K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mDrc7-FLAG

Plasmid

#176470

Purpose

Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

dynein regulatory complex subunit 7 (Drc7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S15A/S78A

Plasmid

#84994

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Serine 15 and 78 mutated to Alanine

Promoter

CMV

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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mIFP12-ILK-C-14

Plasmid

#56255

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

ILK (ILK Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5 FRT TO Myc ArC K72R

Plasmid

#59811

Purpose

Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Mutation

K72R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKR19:SSX1p-mK2-Ex1-(5'D)-ID-BD3-S-(30bp 50%GC)-(linker1)-pA

Plasmid

#251277

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR21:SSX1p-mK2-Ex1-(5'D)-ID-BD4-S-(30bp 30%GC)-(linker1)-pA

Plasmid

#251279

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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