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Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorInsertIgK-IFN (IFNB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
UseTagsExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_HTNV_GP
Plasmid#207281PurposeMammalian expression of Hantaan virus GP proteinDepositorInsertHTNV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-NL63-D14
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC8A3_STOP
Plasmid#161352PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A3 (SLC8A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC8A2_STOP
Plasmid#161340PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A2 (SLC8A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0405
Plasmid#143212PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 KBTBD4 WT
Plasmid#184624PurposeDoxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cellsDepositorInsertN-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationno mutationPromoterTRE promoter, Tet ONAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorInsertRNASEH1 (nuclear isoform) (RNASEH1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-VCPIP1
Plasmid#22592DepositorInsertVCPIP1 (VCPIP1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
INCENP
Plasmid#39185PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertINCENP (INCENP Human)
UseTagsGST-TevExpressionBacterialMutationComplex with AURKBA-c004PromoterAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-EGFP-WPREpA
Plasmid#84469PurposepAAV-RAM-EGFPDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-kanMX6
Plasmid#39294DepositorInsertKanR
UseYeast genomic targetingTags13Myc, A. gossypii translation elongation factor …ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-FOP-dGFP-reporter
Plasmid#14885DepositorInsertmutant LEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationmutant TCF/LEF binding sitesPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG153 FKBP-CFAST11
Plasmid#130813PurposeExpresses FKBP-CFAST11 in mammalian cellsDepositorInsertFKBP-CFAST11
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL
Plasmid#218759PurposeProvides AND intersectional (Cre+Flp) expression of HA-hM4DiDepositorInserthM4Di
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMT3_SRSF1
Plasmid#201056PurposeExpress sumo-tagged human SRSF1DepositorInsertSRSF1 (SRSF1 Human)
UseTagssumoExpressionBacterialMutationnonePromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh
Plasmid#68853PurposeExpresses SMASh tag fused to YFP at C terminus in mammalian cellsDepositorInsertYFP-SMASh
UseTagsSMASh tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP PRDM14
Plasmid#193676PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-spikeRBD::∆ACE2 SURF
Plasmid#206957PurposeFluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2DepositorUseTagscSURF, mCherry, and nSURFExpressionMammalianMutation17-615 and RBD (319-541)PromoterCMVAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Col1a-3'probe
Plasmid#20731DepositorInsertCol1a-3'probe (Col1a1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAGGS-EGFP
Plasmid#22212PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertCAGGS-eGFP
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-METTL3-PURO
Plasmid#174218PurposeExpresses dCasRx-METTL3 in mammalian cellDepositorInsertdCasRx-METTL3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLP3
Plasmid#64946PurposeFlp recombinase expression vectorDepositorInsertFlp recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRX-IB-HaloTag7-mGFP-KDEL
Plasmid#184904PurposeStable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activityDepositorInsertKDEL
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas12a
Plasmid#124891Purposeobtaining full Cas12a RNPDepositorInsertCas12a
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVblast_SLC38A2_wt
Plasmid#156180PurposeCMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.DepositorInsertSLC38A2 (SLC38A2 Human)
UseTagsExpressionMammalianMutationSilent mutations to prevent targeting by sgRNA se…PromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Hygro DEST (w530-1)
Plasmid#19066Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, HygroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only