pSMT3_SRSF1
(Plasmid
#201056)
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PurposeExpress sumo-tagged human SRSF1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSMT3
- Backbone size w/o insert (bp) 744
- Total vector size (bp) 6000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSRSF1
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Alt nameASF1/SF2
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SpeciesH. sapiens (human)
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Insert Size (bp)744
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Mutationnone
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Entrez GeneSRSF1 (a.k.a. ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a)
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Tag
/ Fusion Protein
- sumo (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMT3_SRSF1 was a gift from Jun Zhang (Addgene plasmid # 201056 ; http://n2t.net/addgene:201056 ; RRID:Addgene_201056) -
For your References section:
Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions. Fargason T, De Silva NIU, King E, Zhang Z, Paul T, Shariq J, Zaharias S, Zhang J. Elife. 2023 Mar 2;12:e84412. doi: 10.7554/eLife.84412. 10.7554/eLife.84412 PubMed 36862748
