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pHR-HOXD13-IDR-YFP-NLS

Plasmid

#145277

Purpose

Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet2 enhancer H1

Plasmid

#63883

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 enhancer fragment H1

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

DHFR F[1,2]

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3/FKBP12-pBirA(79-C)-ha

Plasmid

#154449

Purpose

expresses FKBP12-pBirA(79-C)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

FKBP12-pBirA(79-C)-ha (FKBP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

Plasmid

#176588

Purpose

Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associate IscB (Delaware Bay) locus

Use

Tags

Expression

Bacterial

Mutation

Replaced spacer in CRISPR array with Fn spacer

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-hspB3

Plasmid

#63094

Purpose

Expression of HSPB3 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb3 (HSPB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-hspB2

Plasmid

#63093

Purpose

Expression of HSPB2 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb2 (HSPB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSN672

Plasmid

#177362

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Use

Tags

His tag and Leucine zipper

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-DN-RFX

Plasmid

#52296

Purpose

Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

Depositor

Christopher A Walsh

Article

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Insert

Dominant negative RFX

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

Plasmid

#119864

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

HA-tagged biotin ligase (BirA), Thosea asigna vir…

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(+10A)-IDR-mCherry

Plasmid

#145287

Purpose

Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Use

Tags

Expression

Bacterial

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB3

Plasmid

#63104

Purpose

Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb3 (HSPB3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EOMESwt-IDR

Plasmid

#145231

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMESwt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMESwt-IDR (EOMES Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41wt-IDR

Plasmid

#145229

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41wt-IDR (BHLHE41 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13wt-IDR

Plasmid

#145240

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-T0-eGFP-HROB

Plasmid

#135299

Purpose

Expresses eGFP-tagged HROB in mammalian cells

Depositor

Daniel Durocher

Article

Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

Insert

HROB (HROB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TRBV16

Plasmid

#123451

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV16 (TRBV16 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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p-OiRS3GG

Plasmid

#98589

Purpose

Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Inserts

group I intron
PheS cassette to be selectively replaced by unique asRNA probes

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD and pLtetO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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