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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E/D481V + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Tags

Expression

Mutation

D481V + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA51

Plasmid

#174764

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E with STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA49

Plasmid

#174762

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon

Use

Unspecified

Tags

Expression

Mutation

WT + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJH53_pE12_hyCal2

Plasmid

#160408

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal2

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH54_pE12_hyCal7

Plasmid

#160409

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal7

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH55_pE12_hyCal9

Plasmid

#160410

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 hyCal9

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH120_pE12_HyPep_emGFP

Plasmid

#160406

Purpose

Gateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySyn

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyPep emGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH161_pE12_HyPep_mRuby

Plasmid

#160407

Purpose

Gateway entry clone containing HyPep presynaptic component (Hydra RFamide) for HySyn

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyPep mRuby

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E38: attB4-T7p>-attB1r

Plasmid

#162929

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E40: attB4-EFSp>-attB1r

Plasmid

#162928

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

short form of human EF1 alpha promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E34: attB4-mCMVp>-attB1r

Plasmid

#162926

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

minimal CMV IE1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E07: attB2r-SV40polyA-attB3

Plasmid

#162917

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 polyadenylation sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E24: attB2r-Strep2-attB3

Plasmid

#162913

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C231-E02: attB2r-NanoLuc-attB3

Plasmid

#162901

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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