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Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorInserteEF1A1 (EEF1A1 Human)
UseLentiviralTagsFlag-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AHSG-bio-His
Plasmid#52175PurposeExpresses full-length Alpha-2-HS-glycoprotein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAHSG (AHSG Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP
Plasmid#198389PurposeGCN1 R2312A expressonDepositorInsertGCN1 (GCN1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationR2312A mutation in RWDBD (RWD binding domain)PromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmKO-imp α
Plasmid#200082PurposeExpresses mKO-tagged human importinα in mammalian cellsDepositorInsertimportin α (KPNA2 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM-Venus
Plasmid#164785PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Gaussia princeps)
UseTagsGLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304 3xFlag-eRF1 G183/184A_IRES-GFP
Plasmid#198394PurposeeRF1 (AAQ mutant) expressionDepositorInserteRF1 (ETF1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationmutated GGQ motif (G183/184A)PromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
APOH-bio-His
Plasmid#52177PurposeExpresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPOH (APOH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
UseTags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1985
Plasmid#49116PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-mRuby3-WPRE
Plasmid#107744PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1986
Plasmid#49117PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
UseTagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-Parkin C431N
Plasmid#46924Purposeexpresses mutant Parkin with YFP tag in mammalian cellsDepositorInsertPark2 (PRKN Human)
UseTagsEYFPExpressionMammalianMutationchanged cysteine 431 to asparaginePromoterCMVAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
CD69-His-bio
Plasmid#52328PurposeExpresses full-length C-type lectin domain family 2 member C ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertCD69 (CD69 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-K51A
Plasmid#155265PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationLysine 51 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-R70A
Plasmid#155264PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationArginine 70 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-LANA-H1.4CTD
Plasmid#157802PurposeExpression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion proteinDepositorInsertmEGFP-LANA-H1.4CTD (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2116
Plasmid#49141PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
BMR1_02g02691-bio-His
Plasmid#108042PurposeExpresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_02g02691
UseTagsratCD4d3+4,biotinylation sequence, 6xHis (C termi…ExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only