-
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD415
Plasmid#178197PurposeRok _Linker overexpressionDepositorInsertrok _linker
UseTagsExpressionBacterialMutationdeletion of residues 75-96PromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD461
Plasmid#178198PurposeRok with a C-terminal His-tag overexpressionDepositorInsertrok 6xHis
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato-2A-mSyp::EGFP
Plasmid#191213PurposeExpresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.DepositorInserttdTomato-T2A-mSyp::EGFP (Syp Mouse)
UsePcagenTagsmSyp::EGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCble
Plasmid#185861PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-HA
Plasmid#183486PurposeTo express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody dection.DepositorInsertClover4-LUMABS-HA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-HIV
Plasmid#183485PurposeTo express the Clover4-LUMABS-HIV sensor for Acquired Immunodeficiency Virus antibody dection.DepositorInsertClover4-LUMABS-HIV
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-DEN1
Plasmid#183487PurposeTo express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.DepositorInsertClover4-LUMABS-DEN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-EBNA1
Plasmid#183488PurposeTo express the Clover4-LUMABS-EBNA1 sensor for Epstein-barr Virus antibody dection.DepositorInsertClover4-LUMABS-EBNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-VCA
Plasmid#183489PurposeTo express the Clover4-LUMABS-VCA sensor for Epstein-barr Virus antibody dection.DepositorInsertClover4-LUMABS-VCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB394
Plasmid#185086PurposeNMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNma111 KKR 9-11 AAA; KRK 28-30 AAAPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorInsertTSG101 (TSG101 Human)
UseTagsGSTExpressionBacterialMutationaa 2-145 onlyPromotertacAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Arkadia
Plasmid#180372Purposeexpressing mouse Arkadia proteinsDepositorInsertArkadia (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTR promoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA5
Plasmid#180366Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA4
Plasmid#180365Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA6
Plasmid#180367Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA3
Plasmid#180364Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-TagRFP-T-ps
Plasmid#178656PurposeExpression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cellsDepositorInsertTagRFP-T with palmitoylation signal at N-terminus
UseLentiviralTagsPalmitoylation signalExpressionMutationPromoterUbcAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA (PD-1 ITIM) fused to mGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only