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Plasmid#55518PurposeLocalization: Tight Junctions, Excitation: 462, Emission: 492DepositorInsertZO1 (TJP1 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDS1U-PKS12
Plasmid#70150PurposeFusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection markerDepositorInsertPKS12 (PKS12 Fusarium graminearum)
UseTagsExpressionYeastMutationPromoterCUP1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-HindIII
Plasmid#87329PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PI(DM)-Rac1(WT)dc1.FLARE
Plasmid#87589PurposePI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Prp2-pRSETA
Plasmid#61042PurposeEncodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.DepositorInsertPrp2
UseTagsHistidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-Ui4-eSIBR
Plasmid#82586Purpose5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAiDepositorInserthuman ubiquitin C promoter followed by intronically expressed eSIBR cassette
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-deltaNLS1
Plasmid#22153DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationdeletion of amino acids 297-304PromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-ING2
Plasmid#13293DepositorInsertING2 (INGX Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-CEBPA237-C-4
Plasmid#55469PurposeLocalization: Nucleus, Excitation: 462, Emission: 492DepositorInsertCEBPA
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_LUC
Plasmid#45290DepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter dme-mir-2a-1
Plasmid#46664DepositorInsertdme-mir-2a-1
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWzl_neo_DEST_flag_BFP
Plasmid#45302DepositorInsertBFP
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
CtkA_full_pET21a
Plasmid#27428DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3E-V5-mTagBFP2 (JDW 871)
Plasmid#224531PurposeA Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2DepositorInsertV5-mTagBFP2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP574
Plasmid#222953PurposeExpresses R120A LoaPDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 120 to AlaninePromoterT7Available sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFW2000
Plasmid#224211PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP580
Plasmid#222959PurposeExpresses R141S K142S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 141 to Serine and Lysine 142 to …PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP579
Plasmid#222958PurposeExpresses R36S K51S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 36 to Serine and Lysine 51 to Se…PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only