Addgene

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pYPQ265_EtA3C

Plasmid

#225146

Purpose

Gateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

EtA3C-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_DlA3G

Plasmid

#225148

Purpose

Gateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

DlA3G-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_LoA3GX1

Plasmid

#225149

Purpose

Gateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

LoA3GX1-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP65

Plasmid

#196803

Purpose

Contains ATG8A promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

AUTOPHAGY-RELATED 8A

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP72

Plasmid

#196810

Purpose

Contains PMR4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

POWDERY MILDEW RESISTANT 4

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p4P1R_EC1.2en-EC1.1p

Plasmid

#213912

Purpose

Entry clone containing Egg cell-specific promoter (EC1.1p) fused to EC1.2 enhancer (EC1.2en). For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

EC1.2en-EC1.1p

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

Expression

Mutation

R262W

Promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Tags

Expression

Mutation

delPYD (I125M-END)

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - G752A in pENTR

Plasmid

#216623

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

G752A

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R673w in pENTR

Plasmid

#216622

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R673W

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - T551M in pENTR

Plasmid

#216621

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

T551M

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R565C in pENTR

Plasmid

#216620

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R565C

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R376H in pENTR

Plasmid

#216619

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R376H

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - W388S in pENTR

Plasmid

#216618

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

W388S

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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BRAF - R726C in pENTR

Plasmid

#216616

Purpose

gateway cloning donor vector containing BRAF ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

BRAF (BRAF Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R726C

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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