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Plasmid#87072PurposeLentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseLentiviralTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only
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APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/UBC9
Plasmid#20082DepositorInsertUBC9 (UBE2I Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-nucGFP
Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF Synaptophysin-mRuby
Plasmid#188980PurposeSynaptic buton markerDepositorInsertSynaptophysin (Syp Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
UseTagsparS segmentsExpressionYeastMutationPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTARA
Plasmid#31491DepositorInsertRNA polymerase
UseTagsExpressionBacterialMutationN823DPromoterAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-TAX1BP1
Plasmid#175761PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1DepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorInsertsgRNA targeting p53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-CaM
Plasmid#193816PurposeCMV overexpression of ChemoG-CaM in mammalian cell linesDepositorInsertChemoG-CaM
UseTagsExpressionMammalianMutationEGFP:E116D, A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Lysosomes-20
Plasmid#56487PurposeLocalization: Lysosome Membrane, Excitation: 485, Emission: 507DepositorInsertLAMP1
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2497
Plasmid#142115PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin B
Plasmid#11249PurposeMammalian expression of human cathepsin BDepositorInsertcathepsin B (CTSB Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only