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Plasmid#118299PurposeExpression of Rat ERK2DepositorInsertERK2 T183AY185F (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationT183AY185FPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAK112
Plasmid#167280PurposeCodA-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB34
Plasmid#122245PurposeLentivirus delivery for stable expression of LbCas12a-VPRDepositorInsertmammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorExpressionMutationPromoterEFsAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMB22
Plasmid#122244PurposeLentivirus delivery for stable expression of AsCas12a-VPRDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsNLS and VPR activatorExpressionMutationPromoterEFsAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 K52R
Plasmid#118298PurposeExpression of Rat ERK2DepositorInsertERK2 K52R (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationK52RPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only