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Plasmid#185873PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pREP41-MCS+
Plasmid#52690PurposeSame as pREP41, with better MCSDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPnmt41Available sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS12-mCherry
Plasmid#25444DepositorInsertmCherry
UseTagsCOX4 PresequenceExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pREP42-MCS+
Plasmid#52691PurposeSame as pREP42, with better MCSDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPnmt41Available sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EG3
Plasmid#185878PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A5C
Plasmid#185872PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP6G-ccdB
Plasmid#83880PurposeDestination vector for integration at yeast URA3 locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP4G-cccdB
Plasmid#83876PurposeDestination vector for integration at yeast Trp locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP5G-ccdB
Plasmid#83879PurposeDestination vector for integration at yeast Leu locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
yomCherry pDRF1-GW
Plasmid#168087PurposeExpresses yomCherryDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AausFP1 pDRF1-GW
Plasmid#168088PurposeExpresses AausFP1DepositorInsertAausFP1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
yotagRFPT pDRF1-GW
Plasmid#168089PurposeExpresses yotagRFPTDepositorInsertyotagRFPT
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316-Cwc25
Plasmid#111343PurposeCwc25 in pRS316(Ura) vectorDepositorInsertCWC25
UseTagsnoneExpressionYeastMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4ARSd
Plasmid#185876PurposeA plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL-Aur1-2GFP-Hygromycin
Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only