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Plasmid#125185PurposeExpresses the VP2del50 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2697PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP2del30
Plasmid#125184PurposeExpresses the VP2del30 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2637PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c2
Plasmid#120873PurposeExpresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.DepositorInsertCas12c2
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AX53_pS.Donor.S1.TS
Plasmid#100290PurposeAAVS1-targeting plasmidDepositorInsert18 bp RFLP (MluI+KspAI)
UseCRISPR and Synthetic Biology; Human targetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)
Plasmid#104119PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseAdenoviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ReaChR-citrine
Plasmid#50956PurposeReaChR-citrine in lentival transfer vector under human synapsin promoterDepositorInsertReaChR-citrine
UseLentiviralTagscitrineExpressionMutationPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1 alpha, and TRE3GAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT0
Plasmid#210548PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Puro-iDEST
Plasmid#75336PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
AV09_pDonor.EP.S1.TS
Plasmid#100297PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AV11_pDonor.EP.S1
Plasmid#100296PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2163
Plasmid#169632PurposeTier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)DepositorInsertOTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
UseTagsExpressionMammalianMutationPromotertetO7-PhCMVmin / PPGKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor mCherry-PA
Plasmid#72596PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.DepositorInsertmCherry-puromycin cassetter
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-167:CDH5-mEGFP
Plasmid#217425PurposeHomology arms and mEGFP sequence for C-terminus tagging of human cadherin 5DepositorInsertCDH5 Homology Arms with mEGFP (CDH5 Human)
UseCRISPR; Donor templateTagsmEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits