-
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ME-Flag
Plasmid#101860PurposeMds1-Evi-1 flag tagged for transfection into mammalian cellsDepositorInsertMds1-Evi-1 (Mecom Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
AAV-GFP/Cre
Plasmid#49056Purposeadeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control
Plasmid#128749PurposeExpresses control gRNADepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorUseTagsExpressionYeastMutationPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-24xGCN4
Plasmid#205157Purposeexpress tdMCP-24xGCN4DepositorInserttdMCP-24xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2m-IRES-mCherry-CAAX
Plasmid#208690PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorInsertNdi-1 (NDI1 Budding Yeast)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-BFP
Plasmid#107722PurposeFor expression of sgRNA from the U6 promoter with BFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPAICS-EGFP
Plasmid#99108PurposeExpresses human PAICS-EGFP fusion in mammalian cellsDepositorInsertPAICS (PAICS Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
UseTags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorInsertH2B tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-GFP
Plasmid#35363DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-Flag-RNF169
Plasmid#74243PurposeExpresses RNF169 in mammalian cellsDepositorInsertRNF169 (RNF169 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX304 Flag-mCherry-eEF1A1_IRES-GFP
Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorInserteEF1A1 (EEF1A1 Human)
UseLentiviralTagsFlag-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-sfCherry11x4-ß-actin
Plasmid#70220PurposeExpresses sfCherry11x4-ß-actin in mammalian cellsDepositorInsertsfCherry11x4-actin (ACTB Human)
UseTagssfCherry11x4ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MRGPRF-Tango
Plasmid#66437PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMRGPRF (MRGPRF Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SauKKHCas9 PE2*
Plasmid#169852PurposeMammalian Expression, sauCas9-KKH based prime editorDepositorInsertSaCas9KKH-N580A-M-MLV
UseTagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianMutationPromotercmvAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMVA2RBS035
Plasmid#121051PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag-JunB
Plasmid#29687DepositorInsertjun B oncogene (JUNB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
UseTagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXP3
Plasmid#153147PurposeExpression of Flag tagged human FOXP3 in mammalian cellsDepositorInserthuman FOXP3 (FOXP3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
-