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Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v1
Plasmid#195138Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-LATS2
Plasmid#172987Purposeconstitutive expression of FLAG-tagged LATS2DepositorInsert3xFLAG-LATS2 (LATS2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX0393
Plasmid#205984PurposeGa0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacerDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM186A
Plasmid#218127PurposeHybrid circuit displaying hsCRBN bait and driving expression of gIIIDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-LATS1
Plasmid#172986Purposeconstitutive expression of FLAG-tagged LATS1DepositorInsert3xFLAG-LATS1 (LATS1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM656
Plasmid#53086PurposeA non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a controlDepositorInsertLactaherin (Mfge8 Mouse)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro
Plasmid#170320PurposeExpresses HA and mCherry tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro
Plasmid#170317PurposeExpresses HA and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3_ mTFP1- Vamp2
Plasmid#201214PurposeAAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminalsDepositorInsertmTFP1 (from addgene #54613)
UseAAVTagsmouse Vamp2ExpressionBacterial and MammalianMutationPromoterTRE3GAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHes1(467)-luc
Plasmid#41723DepositorInsertHes1 Promoter (-467 to +46) (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoter (-467 to +46)PromoterHes1 promoter fragment (-467 to +46)Available sinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRN120
Plasmid#160696PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminatorDepositorInsertsCmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S
UseLuciferase; Gemini viral replicon, plant developm…TagsExpressionMutationPromoterAtUbi10, CmYLCV, and NosAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB5
Plasmid#63106PurposeExpression of HSPB5 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb5 (CRYAB Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_deltaUBA_UBAP2L_mCherry
Plasmid#105287Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2LDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianMutationdeleted amino acids 20-108PromoterTRE promoter, Tet ONAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xms2-2x3’UTR
Plasmid#122946PurposeFor expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-MS2-HBB 3' UTR
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only