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pAAV-VTKS5-GFP

Plasmid

#178713

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE:iRFP670-EFS:Cre-2A-GFP

Plasmid

#68477

Purpose

Inducible lentivirus expressing iRFP670, Cre, and GFP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

iRFP670
Cre-2A-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Doc2A-GFP KI

Plasmid

#131478

Purpose

Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Doc2a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-B-actin

Plasmid

#117814

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct7

Plasmid

#28200

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-274 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP41

Plasmid

#106407

Purpose

P-RAN-GFP41 (RANbody GFP41::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP41

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDOC-K-glmS-GFPfor

Plasmid

#158059

Purpose

Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

Green Fluorescent Protein optimised for excitation with UV light

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

acpP

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO2_F

Plasmid

#146315

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C840T and sequence differs in…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG eGFP-Oct4

Plasmid

#203789

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou5f1 (Pou5f1 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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CAV1-P132L-mEGFP

Plasmid

#27708

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

P132L

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Use

Tags

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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EHD2-T72A-mEGFP

Plasmid

#45934

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pOPTN217-577-EGFP

Plasmid

#68841

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 210-577 only

Promoter

CMV

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only
HR-TERT-SV40-GFP

Plasmid

#71397

Purpose

Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Tags

SV-40 GFP insertion in TERT promotor

Expression

Mutation

Promoter

Endogenous TERT promotor

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-AP1/beta1

Plasmid

#218802

Purpose

Mammalian expression o fhuman AP1 b1

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

AP1 b1 (AP1B1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-C91G-GFP

Plasmid

#78916

Purpose

Mammalian expression of BAP1 with C91G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91G

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pQUAST-mCD8-GFP

Plasmid

#24351

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

CD8-GFP-SV40

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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