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pDEST_EWSR1_C2G_R650G

Plasmid

#26382

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

R650G

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pRKVSV FAT

Plasmid

#50533

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Use

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
3187 pSFFV-neo Bad cDNA ORF

Plasmid

#8756

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

BAD (Bad Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
CLM RP KGF Mut 1

Plasmid

#24166

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Use

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Promoter

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Claspin-BPI

Plasmid

#31633

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 273-492 of claspin

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Mutation

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Tags

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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p944-SWITCH-OVER insert: EF1a-Puro

Plasmid

#217891

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Plasmid

#216404

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56, Glutamine 70, 443 and Asp…

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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