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Plasmid#193529PurposepENTRY clone for NLR Integrated Domain ID#90DepositorInsertID#90
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM34
Plasmid#193528PurposepENTRY clone for NLR Integrated Domain ID#60DepositorInsertID#60
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM31
Plasmid#193525PurposepENTRY clone for NLR Integrated Domain ID#91DepositorInsertID#91
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM30
Plasmid#193524PurposepENTRY clone for NLR Integrated Domain ID#66DepositorInsertID#66
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ292
Plasmid#102686PurposeS. pombe gRNA entry vector with Cas9 - ScLEU2 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ033
Plasmid#98404PurposeEntry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneExpressionMutationNonePromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 W47A-mycDDK
Plasmid#137769PurposeCALCOCO1 LIR mutantDepositorInsertCALCOCO1 W47A
UseTagsmycDDKExpressionMammalianMutationW47APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSExpressionMutationPromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRPa-iSL(MCS1+2)
Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Phleo
Plasmid#48337PurposeTrasgenically expresses firefly luciferase in T. cruzi with phleomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-DAAT-V33G/T242G
Plasmid#159278PurposeAminotransferase variant with near-native catalytic efficiency toward D-tryptophanDepositorInsertEngineered d-alanine aminotransferase
UseTags6x His tagExpressionBacterialMutationValine 33 and threonine 242 have been mutated to …PromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
UseTagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 V142A-mycDDK
Plasmid#137768PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 V142A
UseTagsmycDDKExpressionMammalianMutationV142APromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 G120
Plasmid#123077PurposeGateway entry clone encoding human MAP1LC3B2 G120DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acids 121-125. Stop codon after G12…PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only