Addgene

We narrowed to 827 results for: nanobodies

Showing: 501 - 520 of 827 results

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pOPINVHH_cys_his

Plasmid

#210403

Purpose

For expression of VHH with C-terminal cysteine and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

cys-his6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

his6-flag

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_sortase_his

Plasmid

#210406

Purpose

For expression of VHH with C-terminal sortase and his tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

sortase-his6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pB2AR-miRFP670nano3

Plasmid

#184671

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

B2AR-miRFP670nano3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYTSG

Plasmid

#159419

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

polyhistidine (poly-His)

Expression

Yeast

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb2E7

Plasmid

#184681

Purpose

NIR-Fb to HIV gp41

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb2E7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Use

Tags

Avi and His

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb4EIG

Plasmid

#184683

Purpose

NIR-Fb to E.coli dihydrofolate reductase

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb4EIG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb59H10

Plasmid

#184682

Purpose

NIR-Fb to HIV p24

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb59H10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Gly42TAG

Plasmid

#125265

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Gly42 nanobody

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-ppiB

Plasmid

#202482

Purpose

Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

peptidyl-prolyl cis-trans isomerase (ppiB)

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

ugpB

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(158-238)

Plasmid

#220744

Purpose

GAL4-sfGFP(158-238) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(158-238)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-NIR-FbLAG30

Plasmid

#220746

Purpose

GAL4-NIR-FbLAG30 construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG16

Plasmid

#220739

Purpose

NIR-FbLAG16 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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