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Plasmid#192589PurposeAAV-vector for fluorescent labeling of noradrenergic neuronsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterPRSX8Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)
Plasmid#225753PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRMT1-Halo
Plasmid#175334PurposeProduction of HaloTag fusion proteinDepositorInsertPRMT1 (PRMT1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-mCherry-CAAX
Plasmid#218189PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Flag-humanRBM47
Plasmid#134607PurposePlasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.DepositorArticleInsertFlag-humanRBM47 (RBM47 Human)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo
Plasmid#204669PurposeMammalian expression of membrane-bound Pink Flamindo for cAMP imagingDepositorArticleInsertMyr-Pink Flamindo
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPF0117
Plasmid#130267PurposeLentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFPDepositorInsertbPAC
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterhPGKAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-AVITAG-GG
Plasmid#215802PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAGDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPOTv7-hygro-mNG
Plasmid#179819PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-3HaloTag
Plasmid#179828PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3x HaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT74
Plasmid#204296PurposepJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-iT7
Plasmid#211699PurposeNIS-Seq-compatible lentiviral vector for expressing sgRNAsDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-puro-3Myc:mNG:3Myc
Plasmid#179803PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-PLIN3
Plasmid#222426PurposeTo express PLIN3 with mEGFP tag in mammalian cellsDepositorInsertPLIN3 (PLIN3 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only