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Plasmid#62020Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertSLC38A10 (SLC38A10 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-tetO-CTR1-shRNA
Plasmid#53180PurposeExpresses shRNA against human CTR1 from puromycin resistance retroviral vectorDepositorInsertCTR1 (SLC31A1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1C389S-HLx8
Plasmid#108140PurposeInactive SAC1 with extended linkerDepositorInsertmCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S-HLx8
Plasmid#108138PurposeRecruitable inactive SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MYL6_EF-hand
Plasmid#109794PurposeProtein expression and purification of MYL6_EF-handDepositorInsertMYL6_EF-hand (MYL6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-3XHA-TMEM192
Plasmid#104434PurposeExpresses 3XHA-TMEM192 in mammalian cellsDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3XHAExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VGAT-WPRE-pA
Plasmid#39320DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV; RTagsExpressionMutationPromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-2XFLAG-TMEM192
Plasmid#104435PurposeExpresses 2XFLAG-TMEM192 in mammalian cellsDepositorInsertTMEM-192 (TMEM192 Human)
UseLentiviralTags2XFLAGExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK24] pACYCDuet His-c7orf59|HBXIP
Plasmid#136143Purposebacterial expression of c7orf59 and HBXIPDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LAMP1-FLAG
Plasmid#71868PurposeoverexpressionDepositorInsertLAMP1 (LAMP1 Human)
UseTagsFLAGExpressionMammalianMutationPromotercmvAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro
Plasmid#208399Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro
Plasmid#208398Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsE2-CrimsonExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro
Plasmid#208400Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0-mC-G-CL3B
Plasmid#124975Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald MAP1LC-C
Plasmid#54151PurposeLocalization: Microtubules, Excitation: 487, Emission: 509DepositorInsertMAP1LC (MAP1LC3B2 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-autophagosome-lysosome reporters
Plasmid#196135Purposezebrafish expressionDepositorInsertautophagosome-lysosome reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-F-actin-microtubule plus ends reporters
Plasmid#196134Purposezebrafish expressionDepositorInsertF-actin-microtubule plus ends reporters
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
vGAT-pH pcaggs SE
Plasmid#78578Purposeimage recycling of VGAT in live neuronsDepositorInsertvGAT-pHluorin C-term (Slc32a1 Rat)
UseTagssuperecliptic pHluorinExpressionMammalianMutationPromoterchicken actinAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWZLblasti-CTR1
Plasmid#53157PurposeExpresses human CTR1 from blasticidin resistance retroviral vectorDepositorInsertCTR1 (SLC31A1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only