Purpose
Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
Insert
ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
Use
CRISPR and Synthetic Biology
Tags
1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
Expression
Bacterial
Mutation
Recoding of BsaI site in the AmpR cassette, two B…
Promoter
lacUV2 driving ω-dCas9 and UV5 driving sgRNA
Available since
April 21, 2021
Availability
Academic Institutions and Nonprofits only