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Showing: 1251 - 1300 of 5798 results

  7. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  15. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

    Plasmid
    #232722
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Insert
    evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    within evoAPOBEC1: H47E+S48A; within Cas9 D10A
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232722-plasmid-map-sequence-id-463545

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  17. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  18. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  27. pHR-IDR4-mCh-Cry2WT-NLS

    Plasmid
    #221920
    Purpose
    Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR4 (aa 662-787) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR4 region (aa 662-787); insert…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221920-plasmid-map-sequence-id-437714

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  28. pHR-CFID-mCh-Cry2WT-NLS

    Plasmid
    #221923
    Purpose
    Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP CFID (aa 1892-2441) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the CBP-FUS interaction domain (CFID…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221923-plasmid-map-sequence-id-437710

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  29. pHR-IDR3-mCh-Cry2WT-NLS

    Plasmid
    #221919
    Purpose
    Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR3 (aa 441-587) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR3 region (aa 441 - 587); inse…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221919-plasmid-map-sequence-id-437708

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  30. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid
    #221922
    Purpose
    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR7 region (aa 2109 - 2434); in…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221922-plasmid-map-sequence-id-437705

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  31. pHR-IDR6-mCh-Cry2WT-NLS

    Plasmid
    #221921
    Purpose
    Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR6 (aa 1856-2090) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR6 region (aa 1856-2090); inse…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221921-plasmid-map-sequence-id-437701

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  32. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid
    #221918
    Purpose
    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP IDR1 (aa 72-175) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR1 region (aa 72 - 175); inser…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221918-plasmid-map-sequence-id-437702

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  33. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid
    #219702
    Purpose
    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6:PhiC31-NLS:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219702-plasmid-map-sequence-id-435732

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  36. pAAV-2xflox-BRX-eGFP-NLS

    Plasmid
    #217535
    Purpose
    AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    4X BRE reporter and miniXon casette (Smad1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    BMP response element (BRE)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217535-plasmid-map-sequence-id-429503

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  50. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid
    #196091
    Purpose
    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 31-65 (SAP domain) of mouse S…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196091-plasmid-map-sequence-id-384675

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Showing: 1251 - 1300 of 5798 results