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Showing: 1251 - 1300 of 2028 results

  11. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  16. SNV-gfp-IRES-MCS

    Plasmid
    #53122
    Purpose
    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
    Depositor
    Jeanette Hyer
    Article
    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1
    Type
    Empty backbone
    Use
    Retroviral; For expression in chicken cells
    Tags
    EGFP-IRES
    Expression
    Mutation
    Promoter
    SNV (spleen necrosis virus)
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    53122-plasmid-map-sequence-id-82652

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  30. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

    Plasmid
    #205052
    Purpose
    Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli
    Depositor
    Allie Obermeyer
    Article
    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021
    Insert
    GFP(-6)-H2_mCherry(-6)-L3
    Use
    Tags
    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    205052-plasmid-map-sequence-id-405667

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  33. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

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  34. pET11a-Z-N-DiB2

    Plasmid
    #168473
    Purpose
    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2 + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168473-plasmid-map-sequence-id-332731

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  40. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-372914

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  41. pANAP

    Plasmid
    #48696
    Purpose
    allows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  44. pCAG-NLS-HA-Dre

    Plasmid
    #51272
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    NLS-HA-Dre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51272-plasmid-map-sequence-id-334322

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  46. pNAS1B

    Plasmid
    #61968
    Purpose
    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    mCherry
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Serine 65 to Glycine (GGT), Serine 72 to Alanine …
    Promoter
    PBAD and PLtetO
    Available since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61968-plasmid-map-sequence-id-101267

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Showing: 1251 - 1300 of 2028 results