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Plasmid#86871PurposeTo express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorInsertSF3B6 (SF3B6 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationamino acids 7-29 deletionPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPTXGARE
Plasmid#68542Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with GARE (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutation5x GARE inserted 190 bp 5' pf ATG (GARE = ta…PromoterOPTX promoter with GAREAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMGC
Plasmid#112812PurposePCR template vector for amplifying gRNA(F+E)-OstRNA fragmentDepositorInsertgRNAcore(F+E)-tRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro NFS1 CD
Plasmid#102978PurposeExpression of NFS1 C381A mutantDepositorInsertNFS1 C381A (NFS1 Human)
UseRetroviralTagsExpressionMammalianMutationC381A catalytic site mutant, Silent mutations to …PromoterAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV FRNK
Plasmid#50534DepositorInsertprotein tyrosine kinase, domain
UseTags2X VSVExpressionMammalianMutationtruncated (NT2186-3268)PromoterCMVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)
Plasmid#86923PurposeTo express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationResidues from 1-89 are deletedPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/hTID1
Plasmid#13745DepositorInserthTid1 siRNA (DNAJA3 Human)
UseRNAiTagsExpressionMutationInverted repeat of 21 bp with 6 nuc. spacer.PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
mVenus-C1-GEFT
Plasmid#84340PurposeExpresses p63 (474 a.a.) tagged with mVenusDepositorInsertGEFT (ARHGEF25 Synthetic, Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLN491 (FuGW-G8p-CCL21-1Pe)
Plasmid#105193PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-CCL21-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ2-GAGA
Plasmid#32857DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis,S-tagExpressionMammalianMutationPDZ2 P(161)NGYGF mutated to H(161)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-N1-PDZ1-GAGA
Plasmid#31651DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
UseTagsHis, S-tagExpressionMammalianMutationchanged P(21)NGYGF changed to H(21)NGAGAPromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-control -- AEB3mRFP1
Plasmid#63851PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAEB3Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
Plasmid#50421PurposepTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD
Plasmid#73306PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
UseTagsinteinExpressionBacterialMutationQ867E-Q939E-C935S-Q1011E-C1007SPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-224N
Plasmid#31634DepositorInsertClaspin (CLSPN Human)
UseTagsGSTExpressionBacterialMutation1st 224 amino acids of claspinPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 T179V
Plasmid#27011DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationT179VPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLIA CRX EnR (CC#145)
Plasmid#15155DepositorInsertCRX (Crx Mouse)
UseRetroviralTagsEngrailed RepressorExpressionMammalianMutationamino acids 7–108, containing the CRX homeoboxPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only