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Plasmid#138025PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-4GC-GFP
UseTagsExpressionBacterialMutationAAA CAG GAG AAAPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 19
Plasmid#138024PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-3AT-GFP
UseTagsExpressionBacterialMutationAAT GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 15
Plasmid#138022PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-3AG-GFP
UseTagsExpressionBacterialMutationAAG GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 13
Plasmid#138021PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-2AC-GFP
UseTagsExpressionBacterialMutationACA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 11
Plasmid#138020PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-2AG-GFP
UseTagsExpressionBacterialMutationAGA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 9
Plasmid#138019PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-2AT-GFP
UseTagsExpressionBacterialMutationATA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 7
Plasmid#138018PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AT-GFP
UseTagsExpressionBacterialMutationTAA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 5
Plasmid#138017PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG-GFP
UseTagsExpressionBacterialMutationGAA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 3
Plasmid#138016PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AC-GFP
UseTagsExpressionBacterialMutationCAA GAG GAG AAAPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT271.361
Plasmid#127846Purposeclone A3A-W98Y CBEs by SapI Golden GateDepositorInsertA3A-W98Y
UseTagsExpressionMutationW98YPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
JK305: pMVP/Blast-DEST
Plasmid#121855PurposepMVP destination vector, empty expression vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JK104: pMVP-DEST
Plasmid#121851PurposepMVP destination vector, empty expression plasmid backboneDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only