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Plasmid#172145PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, P83*, Y184*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#172147PurposeMicroscopy inclusion body assayDepositorInsertpCDF.SUMO-B.(SD4).CD
UseTagsExpressionBacterialMutationPylBCD operon (SUMO tag inserted for PylB; RBS in…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationK198E- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationG160S- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMB206-CT81
Plasmid#165411PurposeBacteria gene circuit on low copy backbone.DepositorInsertCT81 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2b
Plasmid#164964PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2b (LOC565691 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1 WD
Plasmid#163580Purposeexpress Dcp2 ΔH1 WD at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationW50A, D54A, L255APromoterDCP2 promoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-CT67
Plasmid#165406PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT67 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-CT82
Plasmid#165407PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT82 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-IC25
Plasmid#165409PurposeBacteria gene circuit on medium copy backbone.DepositorInsertIC25 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-CT61
Plasmid#165403PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT61 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-CT81
Plasmid#165404PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT81 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3-CT66
Plasmid#165405PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT66 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
UseTagsExpressionBacterialMutationPromoterEc-TTL-P112 and BBa_J23116Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔH1
Plasmid#163576Purposeexpress Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationLysine at position 255 is mutated to AlaninePromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 FL-H1
Plasmid#163583Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationThe first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…PromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnma1b
Plasmid#164952PurposeZebrafish kcnma1b gene CDSDepositorInsertkcnma1b (kcnma1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only