pWSK29-wzzBST
(Plasmid
#172968)
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PurposepWSK29 with Salmonella typhimurium wzzBST gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWSK29
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namewzzB gene from Salmonella enterica serovar Typhimurium
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SpeciesSalmonella enterica serovar Typhimurium
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MutationK198E- please see depositor comments
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms K198E mutation does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWSK29-wzzBST was a gift from Renato Morona (Addgene plasmid # 172968 ; http://n2t.net/addgene:172968 ; RRID:Addgene_172968) -
For your References section:
Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar Typhimurium with macrophages and complement. Murray GL, Attridge SR, Morona R. J Bacteriol. 2006 Apr;188(7):2735-9. doi: 10.1128/JB.188.7.2735-2739.2006. 10.1128/JB.188.7.2735-2739.2006 PubMed 16547065