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Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_RAD21
Plasmid#64056PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21DepositorInsertRAD21 Homology Arms (RAD21 Human)
UseTags3X Flag P2A NeoExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld(FKBP F36V)-GFP
Plasmid#53260PurposepEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld(FKBP F36V)-GFP
UseTagsEGFP, Flag, and HAExpressionMutationFKBP F36VPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CEBPB
Plasmid#64055PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPBDepositorInsertCEBPB Homology Arms (CEBPB Human)
UseTags3X Flag P2A NeoExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GSX2-P2A-tGFP-DTA
Plasmid#161750PurposetGFP plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertturboGFP
UseCRISPRTagsP2A-tGFPExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCH_CREB1
Plasmid#64938PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1DepositorInsertCREB1 Homology Arms (CREB1 Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_LMNA_G2
Plasmid#178090PurposeExpresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.DepositorInsertLMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GABPA
Plasmid#64253PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPADepositorInsertGABPA Homology Arms (GABPA Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCH_ATF1
Plasmid#64689PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1DepositorInsertATF1 Homology Arms (ATF1 Human)
UseTags3X Flag P2A NeoExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
NINJA TC
Plasmid#159274PurposeExpresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).DepositorInsertNINJA TC
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ABL1
Plasmid#23939DepositorInsertABL1 (ABL1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FGFR2
Plasmid#23904DepositorInsertFGFR2 (FGFR2 Human)
UseGateway donor vectorTagsExpressionMutationA52D, T525A (compared to GenBank reference sequen…PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ERBB3
Plasmid#23874DepositorInsertERBB3 (ERBB3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSF1R
Plasmid#23928DepositorInsertCSF1R (CSF1R Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BMPR2
Plasmid#23669DepositorInsertBMPR2 (BMPR2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NEK2
Plasmid#23658DepositorInsertNEK2 (NEK2 Human)
UseGateway donor vectorTagsExpressionMutationInsert encodes truncated protein relative to NP_0…PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FGR
Plasmid#23877DepositorInsertFGR (FGR Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only