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Plasmid#183623PurposePB-10X IRF-minCMV-BFP-Triplex-crAAVS1_8-SV40pADepositorInsertCas12 Guide (crAAVS1_8)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11474
Plasmid#183624PurposePB-STAT6RE-minCMV-BFP-Triplex-crAAVS1_6-SV40pADepositorInsertCas12 Guide (crAAVS1_6)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
UseTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLenti6.3/TO/V5-DEST-mTurquoise2
Plasmid#191010PurposeLentiviral expression vector for mTurquoise2DepositorInsertmTurquoise2
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGolden-Neo
Plasmid#51422PurposeContain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reactionDepositorInsertPGK1 phosphoglycerate kinase 1 (PGK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMN-009
Plasmid#195413PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGolden-Hyg
Plasmid#51423PurposeContain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reactionDepositorInsertPGK-Hyg-pA (PGK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-let7 sponge
Plasmid#35664DepositorInsertU6 let-7sponge
UseTagsExpressionMutationPromoterU6Available sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMN-018
Plasmid#195422PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF10DepositorInsert4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK234_032
Plasmid#123995PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2 3xmiR-122T
Plasmid#35672DepositorInsert3 miR-122 target sites
UseLuciferaseTagsExpressionMutationPromoterHSV-TKAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMN-011
Plasmid#195415PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF3DepositorInsert4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK234_033
Plasmid#123996PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_034
Plasmid#123997PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only