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Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p63RhoGEF580-mVenus-N1
Plasmid#84338PurposeExpresses p63 (580 a.a.) tagged with mVenusDepositorInsertp63RhoGEF (580 aa) (ARHGEF25 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-in
Plasmid#71511PurposeMammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.DepositorInsertConnexin 35
UseTagsHaloTag (internal to sequence)ExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
TR-5'UTR-HP-GFP
Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T MDM2 S166D
Plasmid#16238DepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationSer166 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOPTXcGMPRELUC
Plasmid#68503Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutation3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…PromoterOPTX promoter with cGMPREAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 T134R-pcDNA3.1
Plasmid#118211PurposeExpresses FLAG-OTUB1 T134R mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged threonine 134 to argininePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9753-BEAR-mScarlet-preedited
Plasmid#162994PurposeBEAR control plasmid with split mScarlet and intact 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEIS1B-pSP65
Plasmid#21019DepositorInsertMEIS1B (Meis1 Mouse)
UseIn vitro tntTagsFlagExpressionMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(D977A)-2AeGFP
Plasmid#121962PurposeMammalian expression, Transcription regulation, deactivated AaCas12bDepositorInsertCatalytically deactivated AaCas12b
UseTags3xHA tagExpressionMammalianMutationD977APromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNArodA
Plasmid#149656Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-EGFP-FF2
Plasmid#164922PurposeExpresses EGFP cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR S155A
Plasmid#72702PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationS155APromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Rev
Plasmid#112811PurposegRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only