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Plasmid#154451Purposeexpresses flag-pBirA(1-K283)-FRB/biochemical assayDepositorInsertflag-pBirA(1-K283)-FRB
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9n-Nanog-R
Plasmid#122303PurposeExpresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Nanog (Nanog Synthetic, Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to IleuPromoterT7Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
563aa ELL2 pEF4B
Plasmid#127273PurposeExpresses 563aa human WT ELL2DepositorInsertELL2 truncated at SphI (ELL2 Human)
UseTagsExpressionMammalianMutationContains 564 N-terminal amino acids ELL2, termina…PromoterpEF1aAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2
Plasmid#127274PurposeExpresses human ELL2 with HA tag in the middle that does not disrupt functionDepositorInsertELL2 with HA at XbaI (ELL2 Human)
UseTagsExpressionMammalianMutationHA inserted at XbaI after Met 186PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet138 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet186 to Ileu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133 to Ileu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet331 to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
COOHend ELL2
Plasmid#127255PurposeExpresses the C terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (C-terminal end) (ELL2 Human)
UseOtherTagsExpressionMammalianMutationcontains only the last 389 aaPromoterpEF1aAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet1 to Ileu by QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL2mRNA+polyA
Plasmid#127256PurposeFor in vitro translation of human ELL2 with 30 nt pA tailDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationshortens 3'UTR to 40 bp adds A30 tailPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NH2end ELL2
Plasmid#127252PurposeExpresses the N terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (1-287) (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationcontains only amino acids 1-289PromoterpEF1aAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2
Plasmid#127268PurposeFor in vitro translation of WT ELL2 with HA tag inserted near N-termDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationELL2mRNA+polyA with HA tag inserted at aa12 betwe…PromoterT7Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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