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Plasmid#175470PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationH288APromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c021
Plasmid#175468PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationL281RPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSNA-c001
Plasmid#175466PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).DepositorInsertMSN (MSN Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CEA-N domain residues 1-132 (CEACAM5)-pET30b
Plasmid#174693PurposePlasmid expressing CEA-N domainDepositorInsertCEA-N Domain
UseTagsTEV Cleavage Site and hexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
UseTags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC3
Plasmid#111754PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #3 (residues 613-661)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC1
Plasmid#111756PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationcontains coiled coil #1 (residues 506-555)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L-CC2
Plasmid#111755PurposeBacterial expression for structure determinationDepositorInsertDOT1L (DOT1L Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationContains coiled coil #2 (residues 560-608)PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…TagsExpressionMutationPromoterN/AAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA3174
Plasmid#46880PurposeLentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domainDepositorTypeEmpty backboneUseLentiviralTagsFFKBP12 E31G, R71G, K105E and mycExpressionMutationPromoterTRE-TightAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-Express
Plasmid#174375PurposePlasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatibleDepositorTypeEmpty backboneUseExpression vector for targeted integrationTagsExpressionMutationPromoterpCMP1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA3383
Plasmid#46875PurposeA retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3DepositorInsertsoluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceAug. 13, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGC247
Plasmid#19634DepositorInsertGateway(R1-R2)UseFlp/frt, destination vectorTags4xNLSExpressionWormMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC245
Plasmid#19638DepositorInsertGateway(R4-R3)UseFlp/frt, destination vectorTags4xNLSExpressionWormMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC97
Plasmid#19629DepositorInsertGateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)UseFlp/frt, destination vectorTagsExpressionWormMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only