Showing: 5101 - 5120 of 12974 results
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Plasmid#115654PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pKEW321-MbCas12a-RFP-GFP-array
Plasmid#115661PurposeEncodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1DepositorInsertGFP and RFP array for MbCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPlacAvailabilityAcademic Institutions and Nonprofits only -
pKEW336-MBP-TEV-MbCas12a-33362
Plasmid#115670PurposeExpresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purificationDepositorInsertMbCas12a
UseCRISPRTagsMBP-TEVExpressionBacterialMutationPromoterT7 RNAPAvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP1
Plasmid#113972PurposeSingle short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP2
Plasmid#113973PurposeSingle short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP3
Plasmid#113974PurposeSingle short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pSpCTRE-CD4
Plasmid#114010PurposeDox-inducible Cas9 expression with a CD4 selection markerDepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
sgTrack-Gateway
Plasmid#114011Purpose3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
sgTrack-GFP
Plasmid#114012PurposeEmpty sgRNA expression vector with co-expression of TurboGFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMLS253
Plasmid#114271PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMLS415
Plasmid#114272PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gCAG
Plasmid#114384PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAGDepositorInsertgCAG
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPN10-gCTG
Plasmid#114385PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTGDepositorInsertgCTG
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPN10
Plasmid#114386PurposeEmpty gRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPN10-gDM1d
Plasmid#114387PurposepPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGGDepositorInsertgDM1d
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 5101 - 5120 of 12974 results