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Efg1-ΔDBD-Y450S/Y452S/Y456S

Plasmid

#216410

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y450S/Y452S/Y456S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 450, 452 and 456 to Serine, and …

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y478S/Y482S/Y484S

Plasmid

#216411

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y478S/Y482S/Y484S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 478, 482 and 484 to Serine, and …

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Use

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

TEF

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Dhh1 25-425-EGFP

Plasmid

#201430

Purpose

Bacterial protein expression of Dhh1 25-425

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 25-425 only

Promoter

tac

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2 301-970-EGFP

Plasmid

#201429

Purpose

Bacterial protein expression of Dcp2 301-970

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 301-970 only

Promoter

tac

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dhh1 426-506-EGFP

Plasmid

#201431

Purpose

Bacterial protein expression of Dhh1 426-506

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 426-506 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1 D82-129-EGFP

Plasmid

#201426

Purpose

Bacterial protein expression of Dcp1 del82-129

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

deletion of aa 82-129

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3 1-66-EGFP

Plasmid

#201432

Purpose

Bacterial protein expression of Edc3 1-66

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-66 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp1 82-129-EGFP

Plasmid

#201427

Purpose

Bacterial protein expression of Dcp1 82-129

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 82-129 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Pat1 1-240-EGFP

Plasmid

#201435

Purpose

Bacterial protein expression of Pat1 1-240

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-240 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Edc3 67-282-EGFP

Plasmid

#201433

Purpose

Bacterial protein expression of Edc3 67-282

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 67-282 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Pat1 241-422-EGFP

Plasmid

#201436

Purpose

Bacterial protein expression of Pat1 241-422

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 241-422 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dcp2 1-300-EGFP

Plasmid

#201428

Purpose

Bacterial protein expression of Dcp2 1-300

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 1-300 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Pat1 423-796-EGFP

Plasmid

#201437

Purpose

Bacterial protein expression of Pat1 423-796

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Pat1 (PAT1 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 423-796 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Edc3 283-551-EGFP

Plasmid

#201434

Purpose

Bacterial protein expression of Edc3 283-551

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Edc3 (EDC3 Budding Yeast)

Use

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 283-551 only

Promoter

tac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_101-122-GB1_AG

Plasmid

#148811

Purpose

Bacterial Expression of ScEap1p_101-122

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_101-122 (EAP1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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