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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-128-425-Akap9

Plasmid

#196871

Purpose

Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP-Akap9 (128-425) (Akap9 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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114_pAAV-ProC22-CatCh-GFP-WPRE

Plasmid

#125957

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC22

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/20]

Plasmid

#190525

Purpose

Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Ankyrin-B scFv [N105/17]

Plasmid

#190524

Purpose

Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Ankyrin-B scFv [N105/13]

Plasmid

#190523

Purpose

Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

Plasmid

#111392

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA-EYFP

Use

AAV

Tags

EYFP and Myc

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-Cdk5rap2-CTD

Plasmid

#196854

Purpose

Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPHAGE-CR-NENF-C-TAP

Plasmid

#128511

Purpose

Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

NENF (NENF Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Synonymous silent mutations to F81, Y82, G83 and …

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

Plasmid

#113158

Purpose

An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#190506

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFV. Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IRSp53/BAIAP2 (Homo sapiens) recombinant scFV (BAIAP2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PSD-95 scFv [K28/43]

Plasmid

#190489

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PSD-95 scFv [K28/38]

Plasmid

#190487

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
37_pAAV-ProC40-CatCh-GFP-WPRE

Plasmid

#125974

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC40

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Homer1L/S scFv [L113/130]

Plasmid

#190501

Purpose

Mammalian Expression of Homer1L/S scFV. Derived from hybridoma L113/130.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L/S (Mus musculus) recombinant scFV (Homer1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Kir6.1 K+ channel scFv [N366/60]

Plasmid

#190547

Purpose

Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
88_pAAV-ProA5-CatCh-GFP-WPRE

Plasmid

#125889

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA5

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
137_pAAV-ProA3-CatCh-GFP-WPRE

Plasmid

#125887

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA3

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
78_pAAV-ProA27-CatCh-GFP-WPRE

Plasmid

#125910

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA27

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge