-
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_HD_160-223AA
Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-TET2Puro-gal4DBD-miniVPR-HA
Plasmid#207169PurposeDox Inducible Expression Plasmid for gal4DBD-miniVPR-HADepositorInsertGal4DBD-miniVPR
UseLentiviral and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-tet2puro-gal4DBD-HIFCAD
Plasmid#207173PurposeDox Inducible Expression Plasmid for gal4DBD-HIF-CADDepositorInsertGal4DBD-HIF1a-CAD
UseLentiviral; Dox inducibleTags2x MycExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME rras_Q61R
Plasmid#201057PurposeGateway middle entry vector expressing constitutively actve zebrafish rrasDepositorInsertRRas_Q61R (rras Zebrafish)
UseZebrafish expressionTagsExpressionMutationMutation Q61R, constitutively activePromoternoneAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-SHmOCR
Plasmid#206874PurposeEmpty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusionDepositorTypeEmpty backboneUseTagsHSV + 6xHis and SuperHis (14xHis) + monomeric OCR…ExpressionBacterialMutationPromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:R198P mVenus
Plasmid#210519PurposeExpresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationR198PPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:Apollo-NADP+ mVenus
Plasmid#210518PurposeExpresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mScarlet
Plasmid#207961PurposeGateway entry vector with the mScarlet fluorescent proteinDepositorInsertmScarlet
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKS2ec
Plasmid#130855PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopTagsExpressionMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-DYNLL2
Plasmid#192296PurposeGateway entry vector for an inducible 3xFLAG-DYNLL2DepositorInsertDYNLL2 (DYNLL2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-DEST(r)
Plasmid#190238PurposeGateway destination vector, empty AAV vector backboneDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MKAKU41ec
Plasmid#131014PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH1ec
Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH2ec
Plasmid#131018PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ-NF1-L1-dSpRY
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only