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Plasmid#138081PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-AAV- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA HBB
Plasmid#128120PurposeCo-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HBB + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA RUNX
Plasmid#128122PurposeCo-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA RUNX + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA HEK
Plasmid#128123PurposeCo-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HEK + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ER50-SpCas9-ER50
Plasmid#85448PurposeExpresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cellsDepositorInsertER50-SpCas9-ER50
UseAAVTagsdestabilized domain of estrogen receptor ligand b…ExpressionMammalianMutationPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTCAV-FLEx(loxP)-FlpO
Plasmid#67829PurposeCre-dependent expression of FlpO from CAV2 virusDepositorInsertFlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMP472
Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
#1.4 post-G-SynapShot-mScarlet
Plasmid#214716PurposeExpresses green #1.4 post-SynapShot and mScarletDepositorInsert#1.4 post-G-SynapShot
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
#1.4 pre-G-SynapShot-TagBFP
Plasmid#214717PurposeExpresses green #1.4 pre-SynapShot and TagBFPDepositorInsert#1.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianMutationPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
#1.4 pre-R-SynapShot-TagBFP
Plasmid#214719PurposeExpresses red #1.4 pre-SynapShot and TagBFPDepositorInsert#1.4 pre-R-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CAG/WPREΔU3
Plasmid#190727PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-TagBFP
Plasmid#214721PurposeExpresses green #2.4 pre-SynapShot and TagBFPDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-Myc
Plasmid#214722PurposeExpresses green #2.4 pre-SynapShot and Myc tagDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CMV/WPREΔU3
Plasmid#190726PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLuc-FLAG-CB1
Plasmid#158066PurposeMembrane expression detection of Cannabinoid receptor 1DepositorInsertCB1 (CNR1 Human)
UseAAVTagsGaussia LuciferaseExpressionMammalianMutationcontains 4Kb of 3' UTRPromoterpCAGAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only