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Plasmid#222828PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pHEE-R-hyg5A
Plasmid#222829PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan5A
Plasmid#222830PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar5A
Plasmid#222831PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TA)
Plasmid#61513PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TA mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP
Plasmid#61512PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP, Tom20, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP(TA)
Plasmid#61528PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TA mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iSAM
Plasmid#211495PurposeAAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRaDepositorInsertsUniSAM insert
TET-ON
Neo Resistance
Insulator
UseCRISPR and Synthetic BiologyTagsT2A-mCherry TagExpressionMammalianMutationPromoterEF1a, NA, Splicing acceptor, and TREGV promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
yeast_DualReporter
Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE
Plasmid#166970PurposeCre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.DepositorInsertHomo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
UseAAV and Cre/LoxTagsFLAG, T2A, and mCherryExpressionMammalianMutationPromoterhsynAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plenti-ARE-HSVTK-luc
Plasmid#161789PurposeExpresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markersDepositorInsertsLuciferase-T2A-HSV-TK
mCherry P2A blasticidin
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEF1a and Murine Gclc AREAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR
Plasmid#207805PurposeE-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.DepositorInsertE-NoMi
UseLentiviralTags3xFlag tag, copGFP, mCherry, and nanoluciferaseExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2-PA2-CMV-AB-mCh
Plasmid#160435PurposeExpresses human Amyloid Beta-mCherry in ZebrafishDepositorInsertHuman Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterCMVAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-pur-CAG-Bi-DREADD
Plasmid#159457PurposeAAVS1 targeting donor plasmid with Bi-DREADD expression cassette and puromycin selection geneDepositorInsertBi-DREADD
UseTagsExpressionMammalianMutationfusion protein of hM3Dq-mCherry and HA-KORD seper…PromoterCAGAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
Plasmid#26797DepositorInsertEF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
UseTagsExpressionMammalianMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
UPRT-mCh-Nluc-P2A-neo-UPRT
Plasmid#135015PurposeExpresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locusDepositorInsertsmCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR
UseCryptosporidium expressionTagsmCherry and Nluc-neoExpressionMutationPromoterCryptosporidium actin and enolaseAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2
Plasmid#24419DepositorInsertRS1 (HTR4 Human)
UseMouse TargetingTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only