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pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55648

Purpose

Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Con/Fon-GCaMP6M

Plasmid

#137119

Purpose

Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

Plasmid

#55645

Purpose

Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV5, AAV8, and AAV9

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387A

Plasmid

#60654

Purpose

GFP-Ago2 S387A mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

S387A

Promoter

EF1a

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLPo-Myc

Plasmid

#124641

Purpose

AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

FLPo

Use

AAV and Cre/Lox

Tags

myc tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH1-T2A-PuroR

Plasmid

#162342

Purpose

Lentiviral expression of ATOH1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGolt-mCherry / pGolt3-mCherry

Plasmid

#73297

Purpose

Visualization of Dendritic Golgi Satellite

Depositor

Michael Kreutz

Article

Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

Inserts

ER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Use

Tags

ER export and mCherry

Expression

Mammalian

Mutation

Promoter

CMV immediate early promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-EYFP

Plasmid

#137162

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF Con/Foff hChR2(H134R)-EYFP

Plasmid

#55647

Purpose

Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Short Ef1a

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC364 - pAAV CaMKIIa iRFP

Plasmid

#47903

Purpose

An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-GCaMP6F

Plasmid

#137124

Purpose

Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-iC++-EYFP

Plasmid

#137155

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Fon/Von eYFP

Plasmid

#137164

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV Retrograde and AAV8

Insert

3x-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge