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Plasmid#211759PurposesgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-mCherry
Plasmid#135457PurposeMammalian mCherry reporter to read out FLARE and SPARK activationDepositorInsertmCherry
UseTagsmCherryExpressionMammalianMutationPromoterUASAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU1099
Plasmid#14464DepositorInsertunstable gfp-mut3.1 AAV
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHL 1571
Plasmid#37760DepositorInsertsptet_ompC_gfpAaV
pLac_st3 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 1569
Plasmid#37759DepositorInsertsptet_ompC_gfpAaV
pLac_micC_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLED.GluA2
Plasmid#193017PurposeGluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.ENS
Plasmid#193016PurposeExcitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.NPL
Plasmid#193014PurposeNeuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.CaRPv1
Plasmid#193018PurposeExcitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)DepositorInsertBichromatic calcium indicator (jGCaMP7b and jRGECO1a)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.RAB
Plasmid#193015PurposePhotoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135461PurposeMammalian expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH001
Plasmid#80394PurposeContains aTc inducible Scaffold-mCherry-AAV and TetR genesDepositorInsertmCherry RFP
UseSynthetic BiologyTagsFused by GS linkers to Sh3pep-LZX scaffold protei…ExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL630
Plasmid#167836PurposeEncodes OptoLAC3 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL296
Plasmid#167835PurposeEncodes OptoLAC2 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL659
Plasmid#167838PurposeEncodes OptoLAC2B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TET3G-T2A-Puro
Plasmid#158067PurposeEncodes the Tet3G transactivator and puromycin resistance.DepositorInsertpCAG-TET3G-T2A-Puro
UseAAVTagspuromycin reistance geneExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialMutationPromoterfimSAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4
Plasmid#135460PurposeMammalian expression of uFLARE TF componentDepositorInsertCMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
UseTagsHA, flag,ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA EMX1
Plasmid#128121PurposeCo-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA EMX1 + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only