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pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

Plasmid

#173890

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1927

Plasmid

#163093

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-ATOH7-T2A-PuroR

Plasmid

#162343

Purpose

Lentiviral expression of ATOH7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH7 (ATOH7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCS252

Plasmid

#33207

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCga, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Article

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

Plasmid

#173891

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4*

Plasmid

#71402

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4* (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

After aa126, insertion leads to reading frame shi…

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA (pLP140)

Plasmid

#168461

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

Depositor

Jef Boeke, Matthew Maurano

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD Gi only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Article

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

nEF

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-BFP

Plasmid

#137131

Purpose

Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Expression

Mutation

Promoter

Ef1a

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-EGR1

Plasmid

#52724

Purpose

retroviral expression of human EGR1

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

EGR1 (EGR1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-PSD95-EGFP-WPRE

Plasmid

#133785

Purpose

Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

PSD95-EGFP (Dlg4 Rat)

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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