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Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVTagsExpressionMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
N-cadherin in pCCL-c-MNDU3c-PGK-EGFP
Plasmid#38153DepositorInsertsUseLentiviralTagsExpressionMutationPromoterMNDU3c = LTR promoter for U3 region of myeloproli…Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a
Plasmid#84476PurposeLentiviral construct to express dCas9 fused with Dnmt3aDepositorInsertdCas9-Dnmt3a (DNMT3A Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…PromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 VPR
Plasmid#188509PurposeExpresses FLAG tagged SpdCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PIK3CA_WT
Plasmid#81736PurposeGateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB
Plasmid#207859PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCL6-GFP in Artichoke
Plasmid#169931PurposeOverexpression of Full Length of BCL6-GFP fusionDepositorInsertBCL6 (BCL6 Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STAT3_WT
Plasmid#82235PurposeGateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.DepositorInsertSTAT3 (STAT3 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Coff/Fon DREADD Gq-mCherry
Plasmid#183534PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin S14C
Plasmid#60614PurposeEncodes YFP and NLS tagged beta-actin with the S14C polymerization mutationDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationS14CPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Zeo
Plasmid#207738PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX TRE 3G BFP
Plasmid#128070PurposeLentiviral expression of Tet inducible TagBFPDepositorInsertTagBFP
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-STAT1-mGFP-P2A-Puro
Plasmid#199345Purposeexpress murine STAT1 protein fused with mGFPDepositorInsertsignal transducer and activator of transcription 1 (Stat1 Mouse)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-hphMX4
Plasmid#20758DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-vCre
Plasmid#55638PurposeExpresses vCre in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV8InsertvCre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIU211
Plasmid#44036DepositorInsertAGA1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-Cre
Plasmid#196140PurposeAAV-mediated expression of Cre under the CAG promoter .DepositorInsertCre
UseAAVTagsExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-HAND2
Plasmid#170690Purposedoxycycline-inducible overexpression of HAND2DepositorInsertHAND2 (HAND2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmECFP-1
Plasmid#38772DepositorTypeEmpty backboneUseTagsmECFPExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ETV6_RUNX1
Plasmid#205818PurposeExpress mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(Q61L)
Plasmid#13720DepositorInsertEGFP-Rac1(Q61L) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
p4xSTAT6-Luc2P
Plasmid#35554DepositorInsert4xStat6 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoter4xStat6Available sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
Crop-Seq-BFP-WPRE-TS-hU6-BsmbI
Plasmid#137993PurposeBackbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TCARZ-CBh-GFP-SV40PA-GF3-R4A22
Plasmid#203767PurposeFor production of template RNA for use in PRINT, GFP under CBh promoterDepositorInsertPRINT template RNA for GFP
UseIvt templateTagsExpressionMutationPromoterCBhAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-Flag-HA
Plasmid#101766PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion proteinDepositorTypeEmpty backboneUseTagsFlag-HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCC001
Plasmid#124451PurposeMulti-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targetsDepositorInsertHH-BsaI
UseTagsExpressionYeastMutationgRNA expression cassette from pUDP002 modified to…PromoterTDH3 promoter from S.cerevisiae (already present …Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Golgi-TurboID
Plasmid#200964Purpose3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFPDepositorInsertGolgi-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
Plasmid#60224PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.DepositorInsertsUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS, No promoter, and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lck-FRB-ECFP(W66A)
Plasmid#67902PurposeExpresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)DepositorInsertLck-FRB-ECFP(W66A)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-plasma in pMAX
Plasmid#120401Purposeenables eukaryotic expression of human plasma fibronectinDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_206
Plasmid#96920Purposefor SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkB
Plasmid#39978DepositorInsertTrkB (Ntrk2 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-VSVG
Plasmid#1733PurposeVSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan labDepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-p53-shRNA-427
Plasmid#25636DepositorInsertp53-shRNA-427 (TP53 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only