YCplac22-ENT2
(Plasmid
#26684)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneYCplac22
- Backbone size w/o insert (bp) 4854
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameENT2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneENT2 (a.k.a. YLR206W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13_puc_Rev
- 3′ sequencing primer M13_puc_Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Yeast Replication: CEN4 ARS1
E.coli Replication: ori
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCplac22-ENT2 was a gift from Linda Hicke (Addgene plasmid # 26684 ; http://n2t.net/addgene:26684 ; RRID:Addgene_26684) -
For your References section:
The function of yeast epsin and Ede1 ubiquitin-binding domains during receptor internalization. Dores MR, Schnell JD, Maldonado-Baez L, Wendland B, Hicke L. Traffic. 2010 Jan . 11(1):151-60. 10.1111/j.1600-0854.2009.01003.x PubMed 19903324