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Plasmid#225258PurposeMammalian expression of vPEDepositorInsertvPE
UseCRISPRTagsExpressionMammalianMutationR221K K848A H982A N1317RPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1s
Plasmid#72484PurposeExpress gene of interest under truncated EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ134B2.0
Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER Y537S
Plasmid#49499PurposeExpresses HA-tagged ER Y537S mutant in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationTyrosine 537 to SerinePromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-Citroff1
Plasmid#134304PurposeMammalian expression of inverse-response citrate sensorDepositorInsertCitroff1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-dCas9-KRAB
Plasmid#115545PurposeDoxycycline inducible dCas9-KRAB targeting construct for AAVS1 locusDepositorTypeEmpty backboneUseTagsHAExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE2
Plasmid#87085PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApolipoprotein E2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D2s-L-Venus
Plasmid#19966DepositorInsertD2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
UseTagsFlag and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/BDD-FVIII
Plasmid#41035DepositorInsertB domain deleted FVIII (F8 Human)
UseTagsExpressionMammalianMutationB domain deletionPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pci-EGFP-UPF1-WT
Plasmid#135994PurposeWT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG:GPI-GFP
Plasmid#32601DepositorInsertGPI-GFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)
Plasmid#188375PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-mTurquoise2
Plasmid#98886PurposeProduces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRETDepositorInsertmNeonGreen-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-GFP
Plasmid#183906PurposeExpression of GFP under the human GFAP ABC1D promoterDepositorInsertGFP (GFAP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
perbB2-EGFP
Plasmid#39321DepositorInserterbB2 (ERBB2 Human)
UseTagsegfpExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag1-NS3
Plasmid#17645DepositorInsertHCV NS3 (POLY Hepatitis C virus)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC1-P1A1
Plasmid#202842PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1DepositorInsertEGFP/BC1
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-eGFP
Plasmid#176861PurposeExpresses cytosolic eGFP in astrocytesDepositorInserteGFP
UseAAVTagsExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-i53
Plasmid#92170Purposerecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1DepositorInserti53
UseAAVTagsFLAGExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJuly 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E545K
Plasmid#116485PurposeLentiviral expression of PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE545KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertGja1 (Gja1 Rat)
UseTagsmonomerized superfolder GFPExpressionMammalianMutationPromotercmvAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2h-IRES-mCherry-CAAX
Plasmid#208691PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Ptac
Plasmid#74064PurposeIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA1
Plasmid#153109PurposeExpression of Flag tagged human FOXA1 in mammalian cellsDepositorInserthuman FOXA1 (FOXA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only