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Plasmid#118565PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-167
Plasmid#118579PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLF060: Lenti-CaMKIIa-jRGECO1a
Plasmid#112282PurposeFor lentiviral or direct expression of cytosolic jRGECO1a in excitatory neuronsDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCaMKIaAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-pU6-sgRNA
Plasmid#159174PurposeVector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neuronsDepositorInsertdSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sf-U7snRNA-ESE-AS-NsiI
Plasmid#190694PurposeBasic vector used for the expression of U7-based antisense oligonucleotidesDepositorInsertU7 snRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173863PurposeMammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmVenus-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-hlPYK WT
Plasmid#187975PurposeExpresses the human liver pyruvate kinaseDepositorInserthlPYK WT
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …PromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-HA-FLAG-TIN2
Plasmid#35025DepositorInsertTIN2 (TINF2 Human)
UseAdenoviralTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (LR)-mNeonGreen-IRESpuro2
Plasmid#183743PurposeExpresses Ki67 (LR)-mNeonGreenDepositorInsertMKI67 LR domain (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationLR domain of Ki67PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6020nes
Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 Δ593-618
Plasmid#47895PurposeExpresses myc tagged Miro1 lacking transmembrane domainDepositorInsertMiro1 Δ593-618 (RHOT1 Human)
UseTagsmycExpressionMammalianMutationΔ593-618, lacks transmembrane domainPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-163
Plasmid#118575PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG TIF1gamma M (456-887)
Plasmid#15735DepositorInsertTIF1 gamma M (TRIM33 Human)
UseTagsFlagExpressionMammalianMutationMiddle region (amino acids 456-887).PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only