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Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mCherry-FRB*
Plasmid#207145PurposeLysosome-targeted rapalog dimerizerDepositorInsertLAMP1 (LAMP1 Human)
UseTagsFRB* (T2098L mutant of FKBP-rapamycin binding dom…ExpressionMammalianMutationSilent mutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FlipCherry-T2A-GFP
Plasmid#124436PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cellsDepositorInsertFlipCherry-T2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSK663
Plasmid#205129PurposeExpression of GFP-1xPX (from SnxA) in mammalian cellsDepositorInsert1xPX domain of SnxA
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9 ABE-Umax
Plasmid#222138PurposeAdenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9 (cas9 Synthetic)
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
N370S GBA pcDNA3.1
Plasmid#188583PurposeEctopic expression N370S GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationN370SPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MXS_BidirectionalCAG
Plasmid#78175PurposeBidirectional CAG promoter (compatible with the MXS Chaining Kit)DepositorInsertBidirectional CAG Promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a(+)-PE2
Plasmid#180015PurposeExpressing PE2 protein in E.coli for protein purificationDepositorInsertPE2
UseTags6xHisExpressionBacterialMutationPromoterLac operator-controlled T7 promoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato
Plasmid#213914PurposeAAV construct expressing dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato_nlsdTomatoDepositorInsertdTomato_P2A_nls.dTomato_P2A_nls.dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK527a
Plasmid#110606PurposeBTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLL39
Plasmid#15458DepositorInsert
UseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15p65
Plasmid#27661DepositorInsertTetrahymena telomerase holoenzyme p65
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flamindo2
Plasmid#73938PurposeExpresses biosensor for cAMP in mammalian cellsDepositorInsertFlamindo2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-T7-WspR:G249A
Plasmid#79163PurposeExpresses inactive WspR G249A under a T7 promoterDepositorInsertWspR (wspR P. areuginosa)
UseTagsExpressionBacterialMutationG249APromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
NbV5-mCherry
Plasmid#201474PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorInsertNbV5-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV RasV12 Neo (w108-1)
Plasmid#22259DepositorInsertHRAS G12V (HRAS Human)
UseLentiviralTagsExpressionMammalianMutationG12V mutantPromoterAvailable sinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21TtTERT(195-516)
Plasmid#28172DepositorInsertTetrahymena TERT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Cre
Plasmid#170367PurposeAAV vector expressing Cre under human synapsin promotorDepositorInsertCre
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ALKBH5-dCas9
Plasmid#134783PurposeExpression fusion protein ALKBH5-dCas9 in Mammalian cellsDepositorInsertALKBH5 and inactive dCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3
Plasmid#108103PurposeLentiviral expression plasmid of human SIK3 cDNA with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-TVA(mChr)-W3SL
Plasmid#218754PurposeProvides AND intersectional (Cre+Flp) expression of TVA-mCherryDepositorInsertTVA-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28HistevTtTERT(1-516)
Plasmid#28171DepositorInsertTetrahymena TERT
UseTags6xhistidineExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR2P_P474S_P493N
Plasmid#198695PurposeExpression of human FXR2 protein in E. coliDepositorInsertFXR2P (FXR2 Human)
UseTagsMBPExpressionBacterialMutationChanged proline 474 to serine and proline 493 asp…PromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport SREBP-1c
Plasmid#8883DepositorInsertSREBP-1c (Srebf1 Mouse)
UseTagsExpressionMammalianMutationActive version of SREBP-1c.PromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro
Plasmid#223160PurposeExpression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-lifeDepositorInsertdGFP-P2A-NanoLuc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-MARS-p85(iSH2)
Plasmid#205236PurposeExpresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85betaDepositorUseTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRuby3-MLC-IRES-Neo
Plasmid#222635PurposeReporter for activated NM2DepositorInsertmRuby3-MLC (MYL9 Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-UltraCas12a-6xNLS
Plasmid#209023PurposeLentiviral vector expressing human codon-optimized UltraCas12a with 6xNLSDepositorInsertUltraCas12a-6xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationM537R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT54
Plasmid#218726PurposeExpresses N-terminally EGFP-tagged IFT54 in mammalian cellsDepositorInsertIFT54 (TRAF3IP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AncBE4stem
Plasmid#208766PurposeAncBE4max that co-expresses UGI and p53DDDepositorInsertAncBE4max with 2A-UGI and 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
hsKIF5B(1-560)-HaloTag-FRB
Plasmid#213899PurposeImaging of KIF5B fused to HaloTagDepositorInserthsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DUGV_GP
Plasmid#207268PurposeMammalian expression of Dugbe virus GP proteinDepositorInsertDUGV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only