Addgene

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GfaABC1D-cyto-Ruby3-iATPSnFR1.0

Plasmid

#102554

Purpose

expresses cytoplasmic ATP sensor in astrocytes with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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NIR-AG

Plasmid

#210728

Purpose

NIR light-activated GSDMD

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

NIR-AG (GSDMD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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NsvBa

Plasmid

#100015

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

Depositor

Paul DeCaen, David Clapham

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NsvBa

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin A (CCNA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pegfp-N1-TFEB-S3A,R4A

Plasmid

#44446

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed both Serine 3 and Arginine 4 to Alanine

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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HP138-neo

Plasmid

#134247

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

Contains Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Use

Tags

MBP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

SgRNA expression system

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TRAM1-EGFP

Plasmid

#210365

Purpose

Expresses TRAM1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM1 (TRAM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-H2GH

Plasmid

#182202

Purpose

Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

histone2B-HaloTag-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1 blast tBID-flag

Plasmid

#211533

Purpose

Expresses truncated BID upon doxycycline addition to trigger apoptosis

Depositor

Richard Possemato

Insert

tBID-flag (BID Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated (CASP8) form (p15)

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-memGFP-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-SUN1_916

Plasmid

#125850

Purpose

Expresses SUN1_916 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_916 (SUN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

stop codon

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Tags

Expression

Mutation

Promoter

CAG

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPCK

Plasmid

#133128

Purpose

a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

PEP-carboxykinase gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

PEP-carboxykinase promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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