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hMLKL NB(1-182)-2xFv-Venus

Plasmid

#106077

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL NB(1-182)-2xFv-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Mutation

amino acids 1-182

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Puromycin-mVenus-MAP

Plasmid

#44118

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Venus2-Y705F/S727A-STAT3

Plasmid

#123177

Purpose

Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

Double Y705F and S727A substitutions

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Use

Tags

Venus(156-239)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
AAV-CAG-FLInChR-mVenus

Plasmid

#119298

Purpose

FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

Depositor

Alla Karpova

Article

Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

Insert

Nx1BTM-FCS-ChR2ET/TC-mVenus

Use

AAV

Tags

mVenus

Expression

Mutation

Promoter

CAG

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mVenus

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-tagged Apollo-NADP+

Plasmid

#71797

Purpose

Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Venus1-Y705F/S727A-STAT3

Plasmid

#123176

Purpose

Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Double Y705F and S727A substitutions

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Use

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

metallothionein promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

mVenus-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Venus-Akaluc

Plasmid

#209768

Purpose

Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).

Depositor

Yohei Hayashi

Insert

Venus-Akaluc

Use

Luciferase; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

Plasmid

#137960

Purpose

Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR (non-binding yellow variant)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Y140A binding site mutation

Promoter

CAG

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CIp10 - YemVenus (OPT High)

Plasmid

#163122

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

YemVenus OPT High

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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